A Method for Obtaining Polymorphonuclear Leukocytes from Intraperitoneal Exudates

By modification of existing methods, technics have been developed for obtaining polymorphonuclear leukocytes in good yield from intraperitoneal exudates of guinea pigs. The cells were 80-98 per cent viable at the time of harvest and they maintain this viability for at least 24 hours if stored under refrigeration. Good yield, viability and metabolic activity are dependent on careful handling of the cells and the exclusion of extraneous material from the suspending medium. Since the cells are maintained without extraneous material, they are especially suitable for metabolic studies.