Immunohistochemical Study of Thrombolytic Mechanisms

The indirect immunofluorescent staining technic has been employed to study the localization onto fibrin clots of components of the fibrinolysin system and its activators. Immunodiffusion technics revealed the heterogeneity of the various enzyme preparations used. The activated fibrinolytic enzyme preparations were found to localize onto and diffuse into the matrix and core of the clots. High degree of localization was seen with streptokinase-, urokinase- and spontaneously activated human plasmin, as well as human plasminogen. Chloroform-activated bovine plasmin localized to a lesser extent. No differences were observed in the results whether the fibrin clots were of human, canine or bovine origin.