The addition of increasing levels of phytohemagglutinin to amethopterin-treated cultures of human lymphocytes results in the induction of DNA synthesis which can be measured by the incorporation of exogenous radioactive thymidine. The graded nature of this response permits the assay of mitogenic activity of such preparations as well as the assessment of antimitogenic factors in sera.

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© 1965 by American Society of Hematology, Inc.
1965
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