Euchrysine, A Supravital Fluorescent Lysosomal Stain: Technic and Application for Hematologic Investigation

Euchrysine, a fluorescent aminoacridine dye which is selectively accumulated in lysosomes, has been used in an investigation of cells of hematopoietic origin. The technics for its preparation and use are described as well as the characteristics and patterns of lysosomal fluorescence for 1) primary lysosomes in peripheral blood and bone marrow cells; 2) secondary lysosomes; and 3) lysosomes and lysosomal aggregates in states of altered lymphoid proliferation. Euchrysine fluorescent staining appears to be a convenient and reliable technic for the identification of lysosomes in hematologic investigation.