Brief Report: Transplantation of Allogeneic Canine Bone Marrow Stored at -80 C. in Dimethyl Sulfoxide

Prompt initial bone marrow engraftment was observed in 10 lethally irradiated dogs receiving infusions of 9.8 to 30.0 x 10⁹ allogeneic marrow cells stored at -80 C. in dimethyl sulfoxide. The 3 recipients of bone marrow from unrelated donors, mismatched by canine histocompatibility testing, subsequently rejected their grafts and died within 16 days with marrow hypoplasia. The 3 dogs with matched unrelated donors and the 4 with matched litter mate donors all showed sustained marrow engraftment. Evidence of marrow repopulation by allogeneic cells was obtained by cytogenetic studies in one and by change to donor red cell type in 3 instances.