The Separation of Normal Human Leukocytes by Density and Classification by Size

Leukocytes of hematologically normal human blood have been studied by separating them in bovine serum albumin gradients. The blood cells of the density fractions have been characterized by electronic volume sizing and microscope differential counts. The electronic volumes of different leukocyte populations have been determined from density gradient experiments. Lymphocytes of various sizes can be separated by differences in density. The small lymphocyte population is the most dense while the large population is the least dense. The density of blood cell populations in BSA in order of increasing density were: monocytes, lymphocytes, granulocytes and erythrocytes.