Tryptic peptides characteristic of mouse hemoglobin α- and diffuse-type β-chains were isolated from dimethylsulfoxide-treated mouse leukemia virus-infected cell cultures. Quantitative estimates of in vitro hemoglobin synthesis by such cultures were obtained by two different methods: by analysis of radioactive amino acid incorporation, and by single-cell radial immunodiffusion assay. The amounts produced, approximately 4 pg/cell/17 hr, reflect a synthetic capacity that is probably sufficient for 32P labeling of hemoglobin messenger RNAs (mRNA) in quantities commensurate with structural characterization of mRNAs by radioautographic methods.

Topics:
amino acids, cell culture techniques, dimethyl sulfoxide, globin chain, hemoglobin, hemoglobin biosynthesis, immunodiffusion measurement, leukemia, leukemic cells, mice
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© 1972 by American Society of Hematology, Inc.
1972
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