Friend leukemic cells grown in suspension culture with dimethylsulfoxide can be induced to respond to erythropoietin by increased synthesis of hemoglobin. Investigation was undertaken to determine if these erythroleukemic cells could be induced to form erythroid colonies in a plasma-clot system. This study demonstrates that culture for 24-48 hr in suspension in the presence of 2% dimethylsulfoxide affected these cells so that they then produced benzidine-staining erythroid colonies in the plasma-clot culture. The cells responded to erythropoietin in these cultures with an increase in the number of erythroid colonies.

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© 1974 by American Society of Hematology, Inc.
1974
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