Interleukin-10 (IL-10) is an acid-sensitive protein of 35 kD that has pleiotropic effects including inhibition of cytotoxic T-cell response, induction of major histocompatibility complex type II in B lymphocytes, induction of B-cell growth and differentiation, and autocrine growth factor activity in monocytes. We and others have shown that IL-10 is produced spontaneously by blood mononuclear cells from human immunodeficiency virus-seropositive patients. In an attempt to ascertain the potential role of IL-10 in acquired immunodeficiency syndrome (AIDS)-related B-cell lymphoma, we evaluated the expression of human IL-10 in both tumor-derived B-cell lines and primary tumor cells. Expression of human IL-10 (hIL-10) mRNA and protein was detected in four of five cell lines examined. An IL-10 antisense oligonucleotide inhibited IL-10 mRNA expression and IL-10 protein production. The proliferation of all B-cell lines was inhibited by an antisense oligonucleotide in a dose-dependent manner that was abrogated by the addition of recombinant hIL-10 protein. No effect of antisense oligonucleotide was observed in the B-cell line not producing hIL-10. Evaluation of primary tumor cells from patients with AIDS-lymphoma cells showed similar production and response to IL-10. These data suggest an autocrine growth mechanism for IL-10 in AIDS-related lymphoma cells and that IL-10 may be important in its pathogenesis.
ARTICLES|
June 15, 1995
Interleukin-10 is an autocrine growth factor for acquired immunodeficiency syndrome-related B-cell lymphoma [see comments]
R Masood,
R Masood
Department of Medicine, University of Southern California, Norris Cancer Hospital and Research Institute, Los Angeles 90033, USA.
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Y Zhang,
Y Zhang
Department of Medicine, University of Southern California, Norris Cancer Hospital and Research Institute, Los Angeles 90033, USA.
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MW Bond,
MW Bond
Department of Medicine, University of Southern California, Norris Cancer Hospital and Research Institute, Los Angeles 90033, USA.
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DT Scadden,
DT Scadden
Department of Medicine, University of Southern California, Norris Cancer Hospital and Research Institute, Los Angeles 90033, USA.
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T Moudgil,
T Moudgil
Department of Medicine, University of Southern California, Norris Cancer Hospital and Research Institute, Los Angeles 90033, USA.
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RE Law,
RE Law
Department of Medicine, University of Southern California, Norris Cancer Hospital and Research Institute, Los Angeles 90033, USA.
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MH Kaplan,
MH Kaplan
Department of Medicine, University of Southern California, Norris Cancer Hospital and Research Institute, Los Angeles 90033, USA.
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B Jung,
B Jung
Department of Medicine, University of Southern California, Norris Cancer Hospital and Research Institute, Los Angeles 90033, USA.
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BM Espina,
BM Espina
Department of Medicine, University of Southern California, Norris Cancer Hospital and Research Institute, Los Angeles 90033, USA.
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Y Lunardi-Iskandar
Y Lunardi-Iskandar
Department of Medicine, University of Southern California, Norris Cancer Hospital and Research Institute, Los Angeles 90033, USA.
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Blood (1995) 85 (12): 3423–3430.
Citation
R Masood, Y Zhang, MW Bond, DT Scadden, T Moudgil, RE Law, MH Kaplan, B Jung, BM Espina, Y Lunardi-Iskandar; Interleukin-10 is an autocrine growth factor for acquired immunodeficiency syndrome-related B-cell lymphoma [see comments]. Blood 1995; 85 (12): 3423–3430. doi: https://doi.org/10.1182/blood.V85.12.3423.bloodjournal85123423
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