Human erythrocytes bearing electroinserted full-length CD4 (RBC-CD4) can bind and fuse with a laboratory strain of human immunodeficiency virus type 1 (HIV-1) or with T cells infected by HIV-1. Here we show that RBC-CD4 neutralize primary HIV-1 strains in an assay of cocultivation of peripheral blood mononuclear cells (PBMC) from HIV-1- infected persons with uninfected PBMC. RBC-CD4 inhibited viral p24 core antigen accumulation in these cocultures up to 10,000-fold compared with RBC alone. Viral p24 accumulation was inhibited equally well when measured in culture supernatants or in call extracts. The inhibition was dose-dependent and long-lived. Two types of recombinant CD4 tested in parallel were largely ineffective. The neutralization of primary HIV- 1 by RBC-CD4 in vitro was demonstrated in PBMC cultures from 21 of a total of 23 patients tested at two independent sites. RBC-CD4 may offer a route to blocking HIV-1 infection in vivo.
ARTICLES|
June 1, 1996
Human erythrocytes bearing electroinserted CD4 neutralize infection in vitro by primary isolates of human immunodeficiency virus type 1
PF Tosi,
PF Tosi
Center for Blood Research Laboratory and Harvard Medical School, Boston, MA, USA.
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D Schwartz,
D Schwartz
Center for Blood Research Laboratory and Harvard Medical School, Boston, MA, USA.
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U Sharma,
U Sharma
Center for Blood Research Laboratory and Harvard Medical School, Boston, MA, USA.
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Y Mouneimne,
Y Mouneimne
Center for Blood Research Laboratory and Harvard Medical School, Boston, MA, USA.
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J Hannig,
J Hannig
Center for Blood Research Laboratory and Harvard Medical School, Boston, MA, USA.
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G Li,
G Li
Center for Blood Research Laboratory and Harvard Medical School, Boston, MA, USA.
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G McKinley,
G McKinley
Center for Blood Research Laboratory and Harvard Medical School, Boston, MA, USA.
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M Grieco,
M Grieco
Center for Blood Research Laboratory and Harvard Medical School, Boston, MA, USA.
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CW Flexner,
CW Flexner
Center for Blood Research Laboratory and Harvard Medical School, Boston, MA, USA.
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J Lazarte,
J Lazarte
Center for Blood Research Laboratory and Harvard Medical School, Boston, MA, USA.
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D Norse,
D Norse
Center for Blood Research Laboratory and Harvard Medical School, Boston, MA, USA.
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C Nicolau,
C Nicolau
Center for Blood Research Laboratory and Harvard Medical School, Boston, MA, USA.
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DJ Volsky
DJ Volsky
Center for Blood Research Laboratory and Harvard Medical School, Boston, MA, USA.
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Blood (1996) 87 (11): 4839–4844.
Citation
PF Tosi, D Schwartz, U Sharma, Y Mouneimne, J Hannig, G Li, G McKinley, M Grieco, CW Flexner, J Lazarte, D Norse, C Nicolau, DJ Volsky; Human erythrocytes bearing electroinserted CD4 neutralize infection in vitro by primary isolates of human immunodeficiency virus type 1. Blood 1996; 87 (11): 4839–4844. doi: https://doi.org/10.1182/blood.V87.11.4839.bloodjournal87114839
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