Objective: To study the distribution of CMV glycoprotein B (gB) genotypes in the patients with CMV infection after allo-HSCT

Methods: Seventy-four patients undergoing allo-HSCT were enrolled in this study. After allo-HSCT, all the patients were detected for CMV gB DNA in peripheral blood by haplo-nested PCR methods;The CMV gB DNA positive samples were screened, which were sufficient for enzyme digestion. Glycoprotein B typing was performed through digesting PCR products with RsaIand HinfI;The sequences were mensurated with ABI PRISM 377 XL DNA Sequencer.

Results: The PCR products of thirty-eight patients were digested with enzyme. the distribution of gB genotypes was as follows:I(gB1) 19/38 (50.0%), II(gB2) 3/38 (7.89%),III(gB3) 14/38 (36.84%), and unclassification 2/38 (5.27%).

Conclusion: The distribution of gB genotypes was different in the patients with CMV infection after allo-HSCT; There may be exiting new genotype.

Topics:
allogeneic hematopoietic stem cell transplant, china, cytomegalovirus, genotype, glycoprotein, allopurinol, hematopoietic stem cell transplantation, dna, polymerase chain reaction, cytomegalovirus infections

Author notes

Corresponding author

2005, The American Society of Hematology
2005
Sign in via your Institution