Abstract 3544

Poster Board III-481

Objective

To explore the impact and mechanism of keratinocyte growth factor (KGF) infusion on immune reconstitution post murine allogeneic umbilical cord blood cell transplantation (UCBT).

Methods

Mix cord blood was obtained at E19.5 from several litters of B6 females and was used as umbilical cord blood (UCB) graft. Thirty-two BALB/c mice were randomly assigned to 4 groups with 8 mice per group in the first cohort UCBT, mice were reconstituted with PBS or 1×106, 2×106, 3×106 UCB mononuclear cells (MNCs). Twenty-four BALB/c mice were randomly assigned to 3 groups with 8 mice per group in the second cohort UCBT, mice were injected with 1×106, 2×106 or 3×106 MNCs. All mice received platelet transfusion on +8d. Sixteen BALB/c mice were randomly assigned to 2 groups with 8 mice per group in the third cohort UCBT, mice were injected s.c. with KGF or PBS before TBI. All mice were injected with 2×106 MNCs and were supported with platelet transfusion on +8d. We studied survival, splenic lymphoid cell subsets, sjTREC assay after UCBT.

Results

The survival rates at +100d of mice resconstituted with allogeneic 1×106, 2×106, 3×106 UCB MNCs were 25%, 37.5% and 37.5% in the first cohort UCBT,respectively. The survival rates at +100d of mice injected with 1×106, 2×106, 3×106 MNCs and with platelet support were 87.5%, 100.0% and 100.0%, respectively. The splenic T, NKT, NK and B cell counts in PBS treated control mice on +35d were (9.57±0.74)×106, (0.64±0.06)×106, (1.43±0.10)×106 and (19.13±1.50)×106, respectively. While in KGF treated mice on +35d were (13.47±0.74)×106, (0.89±0.03)×106, (1.79±0.04)×106 and (20.50±0.91)×106, respectively. The level of sjTREC in control mice was 182.2±10.7copies per 105 cells Gwhile that of KGF treated mice was 224.2±9.6 copied per 105 cells and was higher than that of control mice (P=0.019).

Conclusion

Peripheral blood abtained from E19.5d fetus is rich in hematopoietic stem cells. We established a murine allogeneic UCBT model with platelet support on +8d. KGF treatment could enhance immune reconstitution after UCBT.

Disclosures:

No relevant conflicts of interest to declare.

Topics:
cell transplantation, fgf7 protein, human, immune reconstitution, mice, umbilical cord blood, painful bladder syndrome, platelet transfusion, infusion procedures, tissue transplants, mice, inbred balb c

Author notes

*

Asterisk with author names denotes non-ASH members.

© 2009 by The American Society of Hematology
2009
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