Dithizone Staining of Myeloid Granules

A dithizone-acetone-water solution may be used to stain myeloid granules in blood smears and bone marrow preparations. It is necessary to lake the erythrocyte before staining in order to remove interfering substances. The results indicate that zinc, known to be present its leukocytes, is cytoplasmic and not associated with the myeloid granules. It is suggested that the method described may be of value to complement the peroxidase stain as it is rapid, simple, and utilizes solutions which may be stored for lengthy periods.