A number of errors in the figure legends were inadvertently introduced during the publication process. The legend to Figure 4 is duplicated below Figure 5; the legend to Figure 5 is printed as the legend to Figure 6, and the legend to Figure 6 (along with a description of the color key for Figure 7E) is printed as the legend to Figure 7.
Page 892: The legend for Figure 5 should read:
Timed induction of in utero thrombocytopenia. (A) Experimental scheme: mice were injected with anti-GP1bα or IgG control antibodies between E10.5 and E17.5 and analyzed at 6 hours and 48 hours after injection for platelet numbers and hemorrhage phenotypes. (B) Quantification of circulating platelets as a percentage of peripheral blood at 6 hours after injection at E10.5 to E17.5. (C) Images of embryos 6 hours after treatment (E12.5: IgG n = 12, GP1Bα n = 15; E14.5: IgG n = 18, GP1Bα n = 17; E16.5: IgG n = 14, GP1Bα n = 12; E17.5: IgG n = 16, GP1Bα n = 8). Scale bars, 1 mm. (D) Circulating platelets 48 hours posttreatment. (E) E10.5 to E16.5 embryos 48 hours and E17.5 embryos 24 hours posttreatment (E10.5: IgG n = 16, GP1Bα n = 8; E12.5: IgG n = 12, GP1Bα n = 17; E14.5: IgG n = 17, GP1Bα n = 16; E16.5: IgG n = 18, GP1Bα n = 14; E17.5: IgG n = 17, GP1Bα n = 14). Scale bars, 1 mm. (B,D) Data are represented as mean ± SD and analyzed using the Student t test (2-way, unpaired). ****P < .0001. Ab, antibody. Yellow arrows indicate sites of hemorrhage.
Timed induction of in utero thrombocytopenia. (A) Experimental scheme: mice were injected with anti-GP1bα or IgG control antibodies between E10.5 and E17.5 and analyzed at 6 hours and 48 hours after injection for platelet numbers and hemorrhage phenotypes. (B) Quantification of circulating platelets as a percentage of peripheral blood at 6 hours after injection at E10.5 to E17.5. (C) Images of embryos 6 hours after treatment (E12.5: IgG n = 12, GP1Bα n = 15; E14.5: IgG n = 18, GP1Bα n = 17; E16.5: IgG n = 14, GP1Bα n = 12; E17.5: IgG n = 16, GP1Bα n = 8). Scale bars, 1 mm. (D) Circulating platelets 48 hours posttreatment. (E) E10.5 to E16.5 embryos 48 hours and E17.5 embryos 24 hours posttreatment (E10.5: IgG n = 16, GP1Bα n = 8; E12.5: IgG n = 12, GP1Bα n = 17; E14.5: IgG n = 17, GP1Bα n = 16; E16.5: IgG n = 18, GP1Bα n = 14; E17.5: IgG n = 17, GP1Bα n = 14). Scale bars, 1 mm. (B,D) Data are represented as mean ± SD and analyzed using the Student t test (2-way, unpaired). ****P < .0001. Ab, antibody. Yellow arrows indicate sites of hemorrhage.
Page 894: The legend for Figure 6 should read:
Identification of a spatial pattern of ICH. (A) Representative images of E10.5 to E17.5 heads and brains 48 hours after treatment with anti-GP1Bα or IgG (scale bars, 1 mm). (B) Frequency of hemorrhage in the ganglionic eminence (GE) and the cortex of treated E10.5 to E17.5 mice after 48 hours (E10.5: IgG n = 16, GP1Bα n = 8; E12.5: IgG n = 12, GP1Bα n = 17; E14.5: IgG n = 17, GP1Bα n = 16; E16.5: IgG n = 18, GP1Bα n = 14; E17.5: IgG n = 17, GP1Bα n = 14). Total number of mice with ICH is provided in parentheses. (C) Frequency of hemorrhage in the GE and the cortex of treated E10.5 to E17.5 mice after 6 hours (E10.5: IgG n = 8, GP1Bα n = 8; E12.5: IgG n = 9, GP1Bα n = 8; E14.5: IgG n = 7, GP1Bα n = 8; E16.5: IgG n = 7, GP1Bα n = 14; E17.5: IgG n = 6, GP1Bα n = 11). Total number of mice with ICH is provided in parentheses. (D) Representative images of hemorrhage in E14.5 (IgG n = 6, GP1Bα n = 7) and E17.5 (IgG n = 4, GP1Bα n = 6) embryos 6 hours posttreatment (scale bars, 1 mm). Yellow arrows indicate sites of hemorrhage.
Identification of a spatial pattern of ICH. (A) Representative images of E10.5 to E17.5 heads and brains 48 hours after treatment with anti-GP1Bα or IgG (scale bars, 1 mm). (B) Frequency of hemorrhage in the ganglionic eminence (GE) and the cortex of treated E10.5 to E17.5 mice after 48 hours (E10.5: IgG n = 16, GP1Bα n = 8; E12.5: IgG n = 12, GP1Bα n = 17; E14.5: IgG n = 17, GP1Bα n = 16; E16.5: IgG n = 18, GP1Bα n = 14; E17.5: IgG n = 17, GP1Bα n = 14). Total number of mice with ICH is provided in parentheses. (C) Frequency of hemorrhage in the GE and the cortex of treated E10.5 to E17.5 mice after 6 hours (E10.5: IgG n = 8, GP1Bα n = 8; E12.5: IgG n = 9, GP1Bα n = 8; E14.5: IgG n = 7, GP1Bα n = 8; E16.5: IgG n = 7, GP1Bα n = 14; E17.5: IgG n = 6, GP1Bα n = 11). Total number of mice with ICH is provided in parentheses. (D) Representative images of hemorrhage in E14.5 (IgG n = 6, GP1Bα n = 7) and E17.5 (IgG n = 4, GP1Bα n = 6) embryos 6 hours posttreatment (scale bars, 1 mm). Yellow arrows indicate sites of hemorrhage.
Page 895: The legend for Figure 7 should read:
Induction of thrombocytopenia in neonates results in cerebellar hemorrhage. (A) (i) Image highlighting the facial vein of a P1 mouse used to deliver anti-GP1bα and IgG control antibodies. (ii) Representative flow cytometry plots of P1 peripheral blood stained with markers of erythrocytes (TER119) and platelets (CD41). (iii) Quantification of circulating platelets at 6 hours (IgG n = 8, GP1Bα n = 8) and 48 hours (IgG n = 4, GP1Bα n = 4) posttreatment. (B) Representative image of a P1 neonate 48 hours after treatment with anti-GP1bα (n = 7 embryos). Yellow arrows indicate sites of hemorrhage. (C) (i-iii) Representative images of brains 6 hours (IgG n = 8, GP1Bα n = 8) and 48 hours (IgG n = 4, GP1Bα n = 4) posttreatment. Yellow arrows indicate sites of cortical hemorrhage; the blue arrow highlights cerebellar hemorrhage (scale bars, 1 mm). (D) Coronal sections of cerebellum 48 hours posttreatment (bars, 1 mm). (E) Frequency of cerebellar hemorrhage in mice treated at E16.5 (IgG n = 10, GP1Bα n = 16), E17.5 (IgG n = 10, GP1Bα n = 12), or P1 (IgG n = 4, GP1Bα n = 7). (F) Summary of changes in ICH location during embryogenesis. ICHs in the ganglionic eminence, cortex, and cerebellum occur sequentially during development in a thrombocytopenic context.
Induction of thrombocytopenia in neonates results in cerebellar hemorrhage. (A) (i) Image highlighting the facial vein of a P1 mouse used to deliver anti-GP1bα and IgG control antibodies. (ii) Representative flow cytometry plots of P1 peripheral blood stained with markers of erythrocytes (TER119) and platelets (CD41). (iii) Quantification of circulating platelets at 6 hours (IgG n = 8, GP1Bα n = 8) and 48 hours (IgG n = 4, GP1Bα n = 4) posttreatment. (B) Representative image of a P1 neonate 48 hours after treatment with anti-GP1bα (n = 7 embryos). Yellow arrows indicate sites of hemorrhage. (C) (i-iii) Representative images of brains 6 hours (IgG n = 8, GP1Bα n = 8) and 48 hours (IgG n = 4, GP1Bα n = 4) posttreatment. Yellow arrows indicate sites of cortical hemorrhage; the blue arrow highlights cerebellar hemorrhage (scale bars, 1 mm). (D) Coronal sections of cerebellum 48 hours posttreatment (bars, 1 mm). (E) Frequency of cerebellar hemorrhage in mice treated at E16.5 (IgG n = 10, GP1Bα n = 16), E17.5 (IgG n = 10, GP1Bα n = 12), or P1 (IgG n = 4, GP1Bα n = 7). (F) Summary of changes in ICH location during embryogenesis. ICHs in the ganglionic eminence, cortex, and cerebellum occur sequentially during development in a thrombocytopenic context.
The publisher apologizes for the errors, which have been corrected in the online version of the article.
This feature is available to Subscribers Only
Sign In or Create an Account Close Modal