DFP Labeling of Platelets During Recovery from Thrombocytopenia

Rat platelets were labeled with tritiated diisopropylfluorophosphate (³H-DFP) during recovery from acute thrombocytopenia. The results indicated that there was significant labeling of megakaryocytes by ³H-DFP which, in the presence of an increased rate of platelet production, resulted in maintenance of relatively constant values for platelet-bound radioactivity during the period of maximum platelet production and reactive thrombocytosis. Significant random loss of platelets was apparent, and, when a cohort of young platelets was transfused to normal recipients, they were destroyed at a normal rate.