The binding properties of human and rabbit antibodies to antihemophilic factor (AHF, factor VIII) have been compared in experiments that sought the basis for their different specificities and secondary properties. Agarose gel filtration demonstrated the formation of stable complexes of AHF with rabbit anti-AHF but not with human anti-AHF. Measurement of bound radiolabeled rabbit anti-AHF provides a very sensitive assay for the AHF antigen that is present in normal and hemophilic plasmas but that is markedly reduced in plasmas from patients with von Willebrand’s disease.

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© 1972 by American Society of Hematology, Inc.
1972
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