Human plasma-derived protein concentrates intended for clinical use must be treated for viral inactivation to ensure patient safety. This study explored the use of liquid iodine for inactivation of several lipid- and nonlipid-enveloped viruses in an antithrombin III (AT-III) concentrate. Iodine at levels of 0.01% to 0.02% caused between 43% and 94% loss of AT-III activity, as well as degradation of AT-III as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. However, addition of up to 0.1% human albumin protected the AT-III against both inactivation and fragmentation. At albumin levels sufficient to retain greater than 75% of AT-III activity, greater than 6 logs of sindbis, encephalomyocarditis, and vesicular stomatitis viruses, greater than 4 logs of pseudorabies, and greater than 3 logs of human immunodeficiency virus were inactivated. Except with sindbis virus, this represented complete inactivation of all the viruses spiked into the AT-III concentrate.
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July 15, 1995
Iodine-mediated inactivation of lipid- and nonlipid-enveloped viruses in human antithrombin III concentrate
F Highsmith,
F Highsmith
Holland Laboratory, Plasma Derivatives Department, American Red Cross, Rockville, MD 20855, USA.
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H Xue,
H Xue
Holland Laboratory, Plasma Derivatives Department, American Red Cross, Rockville, MD 20855, USA.
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X Chen,
X Chen
Holland Laboratory, Plasma Derivatives Department, American Red Cross, Rockville, MD 20855, USA.
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L Benade,
L Benade
Holland Laboratory, Plasma Derivatives Department, American Red Cross, Rockville, MD 20855, USA.
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J Owens,
J Owens
Holland Laboratory, Plasma Derivatives Department, American Red Cross, Rockville, MD 20855, USA.
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E Shanbrom,
E Shanbrom
Holland Laboratory, Plasma Derivatives Department, American Red Cross, Rockville, MD 20855, USA.
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W Drohan
W Drohan
Holland Laboratory, Plasma Derivatives Department, American Red Cross, Rockville, MD 20855, USA.
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Blood (1995) 86 (2): 791–796.
Citation
F Highsmith, H Xue, X Chen, L Benade, J Owens, E Shanbrom, W Drohan; Iodine-mediated inactivation of lipid- and nonlipid-enveloped viruses in human antithrombin III concentrate. Blood 1995; 86 (2): 791–796. doi: https://doi.org/10.1182/blood.V86.2.791.bloodjournal862791
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July 15 1995
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