The CD79 molecule, comprising two polypeptide chains, mb-1 (CD79a) and B29 (CD79b), is physically associated in the B-cell membrane with immunoglobulin. It transmits a signal after antigen binding and may, therefore, be considered the B cell equivalent of CD3. It appears before the pre-B-cell stage, and the mb-1 (CD79a) chain can still be present at the plasma cell stage. In this report, we describe a new anti-CD79a monoclonal antibody, JCB117, which reacts with human B cells in paraffin embedded tissue sections, including decalcified bone marrow trephines. When tested on a total of 454 paraffin embedded tissue biopsies, gathered from a number of different institutions, it reacted with the great majority (97%) of B-cell neoplasms, covering the full range of B-cell maturation, including 10 of 20 cases of myeloma/plasmacytoma. It is of interest that the antibody labels precursor B-cell acute lymphoblastic leukemia samples, making it the most reliable B-cell marker detectable in paraffin-embedded specimens in this disorder. All neoplasms of T cell or nonlymphoid origin were negative, indicating that antibody JCB117 may be of value to diagnostic histopathologists for the identification of B-cell neoplasms of all maturation stages.
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August 15, 1995
CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples
DY Mason,
DY Mason
University Department of Cellular Science, John Radcliffe Hospital, Oxford, UK.
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JL Cordell,
JL Cordell
University Department of Cellular Science, John Radcliffe Hospital, Oxford, UK.
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MH Brown,
MH Brown
University Department of Cellular Science, John Radcliffe Hospital, Oxford, UK.
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J Borst,
J Borst
University Department of Cellular Science, John Radcliffe Hospital, Oxford, UK.
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M Jones,
M Jones
University Department of Cellular Science, John Radcliffe Hospital, Oxford, UK.
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K Pulford,
K Pulford
University Department of Cellular Science, John Radcliffe Hospital, Oxford, UK.
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E Jaffe,
E Jaffe
University Department of Cellular Science, John Radcliffe Hospital, Oxford, UK.
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E Ralfkiaer,
E Ralfkiaer
University Department of Cellular Science, John Radcliffe Hospital, Oxford, UK.
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F Dallenbach,
F Dallenbach
University Department of Cellular Science, John Radcliffe Hospital, Oxford, UK.
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H Stein
H Stein
University Department of Cellular Science, John Radcliffe Hospital, Oxford, UK.
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Blood (1995) 86 (4): 1453–1459.
Citation
DY Mason, JL Cordell, MH Brown, J Borst, M Jones, K Pulford, E Jaffe, E Ralfkiaer, F Dallenbach, H Stein; CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood 1995; 86 (4): 1453–1459. doi: https://doi.org/10.1182/blood.V86.4.1453.bloodjournal8641453
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August 15 1995
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