Using a microspectrographic technic, reflecting optics and narrow bands of the continuum (5500, 4150 and 2650 Å), the author has obtained pictures of human and rat bone marrow cells in unfixed dry smears as well as in suspensions in vivo; erythroblasts showed different absorption patterns. Violet light microspectrography, specific for heme absorption, allowed us to demonstrate the presence of intranuclear heme granules through various stages of maturation of these cells. This confirmed previous and simultaneous results arrived at by cytochemical and other methods.

The presence of nucleic acids has been demonstrated in neutrophil granulations.

Topics:
bone marrow cells, granules, heme, nucleic acids, process of absorption, erythroblasts, neutrophils, optics
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© 1955 by American Society of Hematology, Inc.
1955
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