The French-American-British subtype acute myelomonocytic leukemia with abnormal eosinophils (FAB AML M4Eo) with pericentric inversion of chromosome 16 is cytomorphologically defined by a myelomonoblastic blast population and abnormal eosinophils. Until now, it remained an open question whether these abnormal eosinophils are part of the malignant clone or an epiphenomenon. We analyzed five cases of AML M4Eo with inv(16) and combined May-Grunwald-Giemsa staining with fluorescence in situ hybridization using yeast artificial chromosome clone 854E2, which spans the inv(16) breakpoint on 16p. In the case of inv(16), three instead of the normal two hybridization signals can be observed both on metaphase spreads and in interphase cells. With this approach, we were able to show inversion 16 in abnormal eosinophils and, therefore, identified them as a part of the leukemic cell population.
ARTICLES|
March 15, 1996
The abnormal eosinophils are part of the leukemic cell population in acute myelomonocytic leukemia with abnormal eosinophils (AML M4Eo) and carry the pericentric inversion 16: a combination of May-Grunwald- Giemsa staining and fluorescence in situ hybridization
T Haferlach,
T Haferlach
Second Medical Department, University of Kiel, Germany.
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M Winkemann,
M Winkemann
Second Medical Department, University of Kiel, Germany.
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H Loffler,
H Loffler
Second Medical Department, University of Kiel, Germany.
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R Schoch,
R Schoch
Second Medical Department, University of Kiel, Germany.
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W Gassmann,
W Gassmann
Second Medical Department, University of Kiel, Germany.
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C Fonatsch,
C Fonatsch
Second Medical Department, University of Kiel, Germany.
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C Schoch,
C Schoch
Second Medical Department, University of Kiel, Germany.
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M Poetsch,
M Poetsch
Second Medical Department, University of Kiel, Germany.
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K Weber-Matthiesen,
K Weber-Matthiesen
Second Medical Department, University of Kiel, Germany.
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B Schlegelberger
B Schlegelberger
Second Medical Department, University of Kiel, Germany.
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Blood (1996) 87 (6): 2459–2463.
Citation
T Haferlach, M Winkemann, H Loffler, R Schoch, W Gassmann, C Fonatsch, C Schoch, M Poetsch, K Weber-Matthiesen, B Schlegelberger; The abnormal eosinophils are part of the leukemic cell population in acute myelomonocytic leukemia with abnormal eosinophils (AML M4Eo) and carry the pericentric inversion 16: a combination of May-Grunwald- Giemsa staining and fluorescence in situ hybridization. Blood 1996; 87 (6): 2459–2463. doi: https://doi.org/10.1182/blood.V87.6.2459.bloodjournal8762459
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