Influence of Magnetic Fe₃O₄ Nanoparticle On the Lymphocyte and Macrophage Function of Mice.

In this paper we investigate effects of magnetic nanoparticle of Fe₃O₄ (MNP-Fe₃O₄) on the murine immune system especially in lymphocyte and macrophage function.

ICR mice were assigned randomly into four groups which were treated with normal saline, the dose of 300mg/kg, 600mg/kg and 1200mg/kg MNP-Fe₃O₄. The mice were killed when they were exposed by intragastric administration after 2 weeks. The ratios of spleen to body weight, lymphocyte transformation rate in spleen suspension, phagocytic index of macrophage in belly cavity were detected.

The ratios of spleen to body weight in all experimental groups were not significantly difference in comparison to the control group(P>0. 05). The lymphocyte transformation rate in spleen suspension in experimental groups were all higher than that of the control group, especially in the media dose group(600mg/kg)(P < 0. 05). And the results of phagocytic index of macrophage in belly cavity indicated that though all experimental groups showed that they enhanced the phagocytic function of macrophage more than the control group, media dose group(600mg/kg) could increase the phagocytic index more than the other two experimental group(P < 0. 05).

It is concluded that suitable dose of MNP-Fe₃O₄ supplement enhance cellular immune function and phagocytic funtion of macrophage of mice.

No relevant conflicts of interest to declare.