In the article by Tournamille et al entitled “Arg89Cys substitution results in very low membrane expression of the Duffy antigen/receptor for chemokines in Fyx individuals,” which appeared in the September 15, 1998, issue of Blood (Vol 92: 2147-2156), a correction should be noted. According to the sequence published in Genbank (accession number AFO55992), nucleotide 319 in theFY*X allele is an adenine and not a guanine. Hence, on p 2150, the sentence beginning “It is noteworthy” should begin “It is noteworthy that all Fybweak cDNA clones carried an A at nucleotide 319 …”. Figure 3 also needs a correction and is printed below in its entirety.
Figure 3. Schematic comparative structure of the DARC cDNAs isolated from whole blood of Fy(a−b+) and Fy(a−bweak) donors. The full-length major isoform (spliced) of the FY transcripts was isolated by RT-PCR. The nucleotide sequences of Fy(a−b+) and Fy(a−bweak) cDNA clones were identical, except for the C286T substitution (+1 taken as the erythroid cap site) resulting in the Arg89Cys polymorphism on the DARC protein. A was found at nucleotide position 319 in all clones from four unrelated Fy(a−bweak) donors, whereas G or A, resulting in an Ala100Thr amino acid change, could be found in the FY*B allele from Fy(a+b+) and Fy(a−b+) donors. The nucleotide residue found at the Fya/Fyb-associated polymorphic position (G146A) is indicated.
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