There are errors in the article resulting from an error in the analysis of the RNA sequencing data. The corrected supplemental Figure 5, which has been corrected online, and corrected Figure 6, shown below, reflect the updated analysis. In the first sentence of the first full paragraph on page 2267, “83 genes” should read “104 genes.” In the second sentence, “n = 430” should read “n = 501.” The errors have been corrected in the online version of the article.
![Altered Vγ9Vδ2-T–cell transcriptional profile in CLL patients. RNA sequencing was performed on paired Vγ9Vδ2-T cells from 4 CLL patients and 4 HCs, directly after thawing and after autologous moDC-based expansion. (A) Multidimensional scaling plot of gene-expression data. Each circle represents an individual donor. (B) Number of genes differentially expressed (false discovery rate < 0.05) in the indicated comparisons. (C) Gene set enrichment analysis of CLL vs HC Vγ9Vδ2-T cells, before (x-axis) and after (y-axis) ex vivo expansion. Each circle represents a gene set [derived from the Hallmark (H) or BioCarta (B) gene sets from the Molecular Signatures Database or self-generated gene sets]. Gene sets differentially expressed (P < .01) before, but not after (P > .1), expansion are highlighted in orange and purple. (D) Gene set enrichment analysis of CLL vs HC Vγ9Vδ2-T cells directly prior to ex vivo expansion. The red dashed line indicates P = .05. Also shown is whether each gene set is up- or downregulated in CLL in comparison with HC Vγ9Vδ2-T cells. (E-F) The gene expression of CLL Vγ9Vδ2-T cells was compared with HC Vγ9Vδ2-T cells. Each circle represents an individual gene. Genes related to synapse and adhesion (E) or inhibitory and exhaustion (F) molecules are highlighted in red. Genes with a P value < .05 and/or a fold change ≤ −1.75 or ≥ 1.75 are annotated. FC, fold change; NK, natural killer cell.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/134/15/10.1182_blood.2019002997/4/m_bloodbld2019002997f6.png?Expires=1735502456&Signature=N7Gmzv1vFRVocFw7S9ElzA6wf6P8RdbPWqmq~7LTmAmUnbGylmeSmZjnJEkemI6eLINDGvHLQP7NjC8MxOQKitmgeuy9pICr-xcn21ls1DnZALVQhHRI0HKMCtVJ2a4XVtgRJ~2ZxnTNmyCw-S7WN8x6VNO5hgaAX4vs85hr7QXQuFz3boQ5J3SDc-7e-X1WQ76UW54C1h-iVd6Ybs0Iuvkuqmmo87GJ~joDgE2vWOhTaqLS1qz1R-C-z8caKMYzjOiPX5ot3UEFcQqKsCq3MpXdlx-U4Fly54-yRbcNLxUO1TmXyXQORH8xMechEvA2hkMYK~nDO3MuqFw1RNE0lQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Altered Vγ9Vδ2-T–cell transcriptional profile in CLL patients. RNA sequencing was performed on paired Vγ9Vδ2-T cells from 4 CLL patients and 4 HCs, directly after thawing and after autologous moDC-based expansion. (A) Multidimensional scaling plot of gene-expression data. Each circle represents an individual donor. (B) Number of genes differentially expressed (false discovery rate < 0.05) in the indicated comparisons. (C) Gene set enrichment analysis of CLL vs HC Vγ9Vδ2-T cells, before (x-axis) and after (y-axis) ex vivo expansion. Each circle represents a gene set [derived from the Hallmark (H) or BioCarta (B) gene sets from the Molecular Signatures Database or self-generated gene sets]. Gene sets differentially expressed (P < .01) before, but not after (P > .1), expansion are highlighted in orange and purple. (D) Gene set enrichment analysis of CLL vs HC Vγ9Vδ2-T cells directly prior to ex vivo expansion. The red dashed line indicates P = .05. Also shown is whether each gene set is up- or downregulated in CLL in comparison with HC Vγ9Vδ2-T cells. (E-F) The gene expression of CLL Vγ9Vδ2-T cells was compared with HC Vγ9Vδ2-T cells. Each circle represents an individual gene. Genes related to synapse and adhesion (E) or inhibitory and exhaustion (F) molecules are highlighted in red. Genes with a P value < .05 and/or a fold change ≤ −1.75 or ≥ 1.75 are annotated. FC, fold change; NK, natural killer cell.
Altered Vγ9Vδ2-T–cell transcriptional profile in CLL patients. RNA sequencing was performed on paired Vγ9Vδ2-T cells from 4 CLL patients and 4 HCs, directly after thawing and after autologous moDC-based expansion. (A) Multidimensional scaling plot of gene-expression data. Each circle represents an individual donor. (B) Number of genes differentially expressed (false discovery rate < 0.05) in the indicated comparisons. (C) Gene set enrichment analysis of CLL vs HC Vγ9Vδ2-T cells, before (x-axis) and after (y-axis) ex vivo expansion. Each circle represents a gene set [derived from the Hallmark (H) or BioCarta (B) gene sets from the Molecular Signatures Database or self-generated gene sets]. Gene sets differentially expressed (P < .01) before, but not after (P > .1), expansion are highlighted in orange and purple. (D) Gene set enrichment analysis of CLL vs HC Vγ9Vδ2-T cells directly prior to ex vivo expansion. The red dashed line indicates P = .05. Also shown is whether each gene set is up- or downregulated in CLL in comparison with HC Vγ9Vδ2-T cells. (E-F) The gene expression of CLL Vγ9Vδ2-T cells was compared with HC Vγ9Vδ2-T cells. Each circle represents an individual gene. Genes related to synapse and adhesion (E) or inhibitory and exhaustion (F) molecules are highlighted in red. Genes with a P value < .05 and/or a fold change ≤ −1.75 or ≥ 1.75 are annotated. FC, fold change; NK, natural killer cell.
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