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Effect of non-pathogen-reduced (NPR) and pathogen-reduced (PR) platelets on thrombin-induced disruption of adherens and tight junctions. Human umbilical vein endothelial cell (HUVEC) monolayers were stained for vascular endothelial cadherin (green), F-actin with phalloidin (red), and DAPI (4′,6-diamidino-2-phenylindole) (blue) in the color images as well as zonula occludens 1 (white) in the black-and-white images after exposure to NPR or PR platelets. Both types of platelets attenuated loss of endothelial junctional proteins, but the NPR platelets were more effective in the first 5 days after exposure. White arrows indicate gaps between endothelial cells. See the article by Nair et al.