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Tomographic reconstruction of the NFAT-activation signal distribution after adoptive transfer of T-cell precursor cells during immune reconstitution. Transgenic T-cell precursor cells were transplanted into a recipient mouse. Bioluminescence data were acquired and the 3-dimensional (3D) distribution of the NFAT-activation signal was calculated. The reconstructed bioluminescence distribution is volume-rendered in a red-yellow color scale along with a surface-rendered image of the mouse's skeleton extracted from the computed tomographic image. Activated cells were clearly localized in the region of the thymus, which was confirmed by subsequent ex vivo measurements. This composite image was prepared by Bradley Beattie and Alexander Klose. See further experimental and imaging details in the article by Na et al on page e18.